Simply place the module into one of the five module bays and read the assay. spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf certifiedLysates are then transferred ®to the assay plate for the detection of Total CHK2 by HTRF reagents. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. STANDARD . Your lab might even have one already. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. • HTRF®-Certified Reader. Plate cells Stimulate cells 3. On the SpectraMax i3x reader, install the HTRF cartridge, and wavelengths will be selected automatically when HTRF is selected as the Optical Configuration. Make sure the setup for Eu3+ Cryptate is used. VICTOR Nivo Multimode Plate Reader. Plate 25 μL of cells in. Make sure the setup for Tb Cryptate is used. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Microplate readers EnVision ® Multimode Plate Reader VICTOR® Nivo™ EnSight® Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ® The EnVision ® multimode plate reader provides exceptional speed,. Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab. For a list of HTRF-compatible readers and set -up recommendations, please visit. COMPONENTS ; STORAGE . Read & analyse. SERIAL DILUTIONS . **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. For more information about HTRF. Read & analyse. Compared to ELISA technology, MSD assays: Require far less sample. The CLARIOstar® Plus is our most flexible multi-mode plate reader. The signals are produced by, converted by, or transmitted through the. Cells in each image are identified by SoftMax Pro Software and cell-by-cellHTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. It accepts all types of microtitration plate. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Taking these considerations into account, Cisbio’s 96w low volume and PKI’s 96w & 384w microplates have been carefully designed to provide the best signal detection environment possible to all HTRF assays. Incubate overnight at RT. Plate 25 μL of cells in. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Remove the plate sealer and read on an HTRF® compatible reader . FOR HTRF CERTIFIED READER For more information about HTRF. 5 EXAMPLE OF MAP PLATE . FOR HTRF CERTIFIED READER. Assay setup for a 384-well low-volume plate. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. HTRF is a proprietary time-resolved fluorescence technology that overcomesThe company offers different HTRF-certified, multidetection microplate readers such as the FLUOstar Omega, POLARstar Omega, CLARIOstar and PHERAstar. As shown in Fig. Example assays are ELISAs, DNA and protein concentration determination, steady-state dose response curves, ADP-Glo™ (Promega), Transcreener™ GDP fluorescent polarization. FOR HTRF CERTIFIED READER For. For fluorescent assays, black plates absorb light and reduce background and crosstalk. Download Setup. For a list of HTRF-compatible readers and set-up recommendations, please visit . Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Smallest and most affordable multimode plate reader in the industry. Read & analyse. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:FOR HTRF CERTIFIED READER. For more information about HTRF. 5 EXAMPLE OF MAP PLATE . Designed to the highest standards to support your drug discovery research, Corning ® and Falcon ® microplates are ideal for drug discovery applications, including assay prep, assay development, and HTS and automation. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit. . Incubate overnight at RT. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. The laser beam passes through the sample well. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Read & analyse. Lysates are then transferred to the assay plate for the detection of total-CDK4 by HTRF. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Lysates are then transferred to the assay plate for the detection of Total H2AX by HTRF ® reagents. + 4 µL HTRF ® pre-mixed antibodies . ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. This protocol gives the cells viability and confluence to be monitored. Microplate Readers and Reader Accessories. + 4 µL HTRF pre-mixed antibodies . Phospho-Rb (Ser780) two-plate assay protocol. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. Read other about this measurement approach here. Homogeneous Time-Resolved Fluorescence (HTRF) . Discover our portfolio of multimode plate readers and radiometric detection solutions, complemented by assays, reagents and consumables which together enable you to increase your lab’s. Table 1. This protocol, HTS designed, allows miniaturization while. For more information about HTRF. Dual-Luciferase® (DLR™) gene assays. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Revvity. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Remove the plate seal and read on an HTRF compatible reader. For more information about HTRF. Make sure the setup for Eu3+ Cryptate is used. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. Conclusion. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The donor and acceptor can be used to label a variety of biomolecules, for applications. FOR HTRF CERTIFIED READER. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). This protocol, HTS designed, allows miniaturization while. It continues to deliver optimal results. It guarantees high level of cAMP accumulation in the cells. PROTOCOL AT A GLANCE. These plates can be read on all HTRF certified readers, thus making their implementation in. culture plate. Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. Lyse cells Read on an HTRF compatible reader . FOR HTRF CERTIFIED READER. 96-well or 384-well small volume (SV) detection. VICTOR® Nivo™ multimode plate reader. At Cisbio we provide microplates that help you discover better. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. 47 seconds for a 1536-well plate. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. and Canaa -8-963-4567. 68. 5 hours before a further 3 washing steps. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Remove the plate seal and read on an HTRF compatible reader. + 4 µL HTRF pre-mixed antibodies. For instance, when assay volumes are. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. It can also be further streamlined to a one-. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. The plate was covered both incubated for one hour at room temperature. and TR-FRET, for example to detect PerkinElmer’s DELFIA, HTRF and LANCE assay technologies Time resolved fluorescence certified for use with HTRF. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED. Read & analyse. For more. For a list of HTRF-compatible readers and set-up recommendations, please visit . ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. e. Plate 1 plate HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Plate 25 μL of cells in. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. For more information about HTRF. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. For more ® information about HTRF compatible readers and for set-up recommendations, please. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Remove the plate sealer and read on an HTRF® compatible reader . An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". The DLR assay is a two step reaction that uses two luciferase enzymes, Firefly and Renilla (Figure 1). Make sure the setup for Tb Cryptate is used. The system provides even higher sensitivity for time-resolved fluorescence (TRF) applications. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Use simpler, faster protocols. These plates can be read on all HTRF certified readers, thus making their implementation in. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. Specialized filters allow for IR fluorescence. Incubate 4h at RT. Read & analyse. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Detection plates from providers not listed on this. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. Synergy NEO2 readers must be appropriately configured for HTRF® readout by setting up the measurement conditions in the Gen5™ Reader Control and Data Analysis Software. best fluorescence polarization plate reader on the market. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. use of a wide variety of plates or sample matrix formats. ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility. Handle complex biological matrices easily. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. FOR HTRF CERTIFIED READER. High Speed Monochromator offers a typical wavelength accuracy of <0. 3-in-1 absorbance using a variety of sample formats, including microplates. The SpectraMax i3x and SpectraMax iD5 readers can be equipped with an HTRF-certified detection cartridge (i3x) or an HTRF-certified module and filters (iD5) to meet the demanding specifications required for HTRF assays. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. Cover the plate with a plate sealer. Homogeneous Time-Resolved Fluorescence (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based) • Kinases and ATPases • Cytokine Assays TUNE Multi-Mode • cAMP Quantitation • Cell Migration • Cell Viability and Proliferation • Cytotoxicity • DNA/RNA Quantitation (Fluorescent) Homogeneous Time-Resolved Fluorescence (HTRF) . SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Fluorescence polarization (FP) is a fluorescence-based detection method that is widely used to monitor molecular interactions in solution. FOR HTRF CERTIFIED. * For HTRF microplate recommendations, please visit . This protocol gives the cells viability and confluence to be monitored. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. pre+ 5 µL HTRFcompounds Activation Buffer. FOR HTRF CERTIFIED. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more information about HTRF ®Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information. 5 μL of Binding buffer 2X. It measures at any wavelength, with top and bottom reading for most standard technologies. Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. • Low volume (20µl) • Compatible with all HTRF readers • For cell-based or biochemical assays Trying is believing. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER . ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. Conclusion. 3. FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Read & analyse. Plate 25 μL of cells in. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER. Configure your microplate reader. These plates can be read on all HTRF certified readers, thus making their implementation in. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. 96-well culture plate• HTRF®-Certified Reader. Remove the plate seal and read on an HTRF compatible reader. * For HTRF microplate recommendations, please visit . They can be used in a no-transfer. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readersFOR HTRF CERTIFIED READER. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Highly compatible plates for HTRF. This protocol. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". Catalog No. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Incubate at room temperature. no. Lysates are then transferred to the assay plate for the detection of total-BTK by HTRF. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. The HTRF cellular assays were found to be at least 16-fold more sensitive than the Western Blot. HTRF® CertifiedCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. HTRF-compatible SpectraMax Multi-Mode Readers. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. 96-well culture plateHTRF certified . Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. acceptor reagent. FOR HTRF CERTIFIED READER. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. In addition, stacker. FOR HTRF CERTIFIED READER. How HTRF can simplify your life The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black plates; Dedicated excitation laser and detectors (PMTs) for best sensitivity; Simultaneous detection of donor and acceptor gives speed and precision See full list on bmglabtech. HTRF is compatible with a large range of available microplate readers. HTRF-specific. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. It delivers even higher sensitivity for time-resolved fluorescence (TRF) applications, and the Enhanced Security software option. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. These plates can be read on all HTRF certified readers, thus making their implementation in. Lysates are then transferred to the assay plate for the detection of Phospho-VASP (Ser239) by HTRF. FOR HTRF CERTIFIED READER. Data Analysis HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. For more information about HTRF ®Highly compatible plates for HTRF. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. It combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background. Your lab might even have one already. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. + 4 µL HTRF pre-mixed antibodies . HTRF certified . CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. FOR HTRF CERTIFIED READER. If not, our scientific team will help you choose the instrument that best suits your needs and budget. Read & analyse. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. Synergy H1 offers researchers a choice of monochromators or filters or both detection technologies in one compact unit. This protocol enables the cells' viability and confluence to be monitored. For more information about HTRF. For more information about HTRF. For HTRF certified readerThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Do not pre-mix the d2 and Cryptate solutions prior to dispensing Make sure to use the set-up for Eu Cryptate. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. If not, our scientific team will help you choose the instrument that best suits your needs and budget. For more. Remove the plate seal and read on an HTRF compatible reader. Seal the plate and incubate 3 hours at RT . -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. PURCHASE SEPARATELY . Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Incubate overnight at RT. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. (In the following text, the term YTHDC1 is used frequently as an abbreviation for the m 6 A-recognition domain of YTHDC1). You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Read & analyse. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. For more information about HTRF ®• HTRF®-Certified Reader. (2) Forskolin activates the adenylyl cyclase enzyme and increases the intracellular level of cAMP. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. To perform the assay, use white plate only. This protocol gives the cells viability and confluence to be monitored. 0. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm:. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. For more. Optimization of the reader for the plate dimensions clearly improved the results as seen by the uniform signal (b), a lowered %CV of 2. This protocol gives the cells viability and confluence to be monitored. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. (donor) of compounds are measured on an HTRF-compatible reader. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. • HTRF®-Certified Reader. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Lysates are then transferred to the assay plate for the detection of total-NFkB by HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . The assay is then ready to be read on an HTRF compatible or certified microplate reader. It enables you to gain insights you couldn’t achieve with detection measurements alone. The FLUOstar Omega is the ideal plate reader for life science applications. FOR HTRF CERTIFIED. All requiredThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more ® information about HTRF compatible readers and for set-up recommendations, please. Even if not listed, some readers may be compatible with HTRF technology. For more information about microplate recommendations, please visit our website STORAGE AND STABILITY• HTRF®-Certified Reader. Transcreener® ADP2 FP assay. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Incubate overnight at RT. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. FOR HTRF CERTIFIED READER. With this certification, Cisbio Bioassays guarantees that the Synergy H4 meets or exceeds strict performance and functional criteria for optimal HTRF® readout. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. For HTRF readings, a 620/40 nm filter (PerkinElmer 2100-5760) and a 665/7. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. After an overnight incubation, the plate is washed 3 times. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **.